FAK/PTK2 Kinase Assay Service

Activity-based FAK/PTK2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

FAK/PTK2

Enzyme

Human FAK/PTK2

HGNC

PTK2

Synonym

FAK, FADK

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	34

Scientific Information

Q05397 (http://www.uniprot.org/uniprot/Q05397)

Screening Location

Malvern, USA

Reference compound IC50 for FAK/PTK2