FER Kinase Assay Service - Reaction Biology

Activity-based FER kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

FER

Enzyme

Human FER

HGNC

FER

Synonym

TYK3, c-FER, p94-FER

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	1.2

Scientific Information

P16591 (http://www.uniprot.org/uniprot/P16591)

Screening Location

Malvern, USA

Reference compound IC50 for FER