Activity-based FGFR1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FGFR1
Enzyme
Human FGFR1, wt
HGNC
FGFR1
Manning ID
FGFR1
Synonym
FGF-R1;H2, H3, H4, H5, CEK, FLG, FLT2, KAL2, BFGFR, C-FGR, N-SAM, FGFR1, H2, FGF-R1 wt
UniProt
P11362 (FGFR1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 26 |
| Sorafenib | 1500 |
| Sunitinib | 1900 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FGFR1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – FGFR1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0101-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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