Activity-based FGFR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR1
Enzyme
Human FGFR1
HGNC
FGFR1
Synonym
H2, H3, H4, H5, CEK, FLG, FLT2, KAL2, BFGFR, C-FGR, N-SAM
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.2 |
| PP2 | 21,000 |
| AG1478 | 25,000 |
| D-64406 | >25,000 |
Scientific Information
P11362 (http://www.uniprot.org/uniprot/P11362)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR1
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