Activity-based FGFR1 V561M kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FGFR1 V561M
Enzyme
Human FGFR1 V561M, mutant
HGNC
FGFR1 (V561M)
Manning ID
FGFR1
Synonym
FGFR1, FGFR-1, bFGF-R, FLT2, CEK, CD331, FGF-R1 V561M, H2, H3, H4, H5, FLG, KAL2, BFGFR, C-FGR, N-SAM, FGFR1 V561M, FGFR1(V561M)
UniProt
P11362 (FGFR1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 25 |
| Sorafenib | 4000 |
| Sunitinib | 290 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FGFR1 V561M |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – FGFR1 V561M (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0890-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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