Activity-based FGFR2 (C491A/V564I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR2 (C491A/V564I)
Enzyme
Human FGFR2 (C491A/V564I)
HGNC
FGFR2 (C491A/V564I)
Synonym
Fibroblast growth factor receptor 2 [Precursor], Keratinocyte growth factor receptor 2
Scientific Information
P21802 (http://www.uniprot.org/uniprot/P21802)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.0 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 36,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for FGFR2 (C491A/V564I)
