FGFR2 (R612T) Kinase Assay Service

Activity-based FGFR2 (R612T) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FGFR2 (R612T)

Enzyme
Human FGFR2 (R612T)

HGNC
FGFR2 (R612T)

Synonym
BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, KGFR, KSAM, K-SAM, TK14, TK25

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.5
PP2 92
AG1478 174
PDGFR I-III >100,000
D-64406 700
Scientific Information
P21802 (http://www.uniprot.org/uniprot/P21802)

Screening Location
Malvern, USA

Reference compound IC50 for FGFR2 (R612T)
Reference compound IC50 for FGFR2 (R612T)

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