FGFR3 (V555M) Kinase Assay Service

Activity-based FGFR3 (V555M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FGFR3 (V555M)

Enzyme
Human FGFR3 (V555M)

HGNC
FGFR3 (V555M)

Synonym
ACH, CD333, CEK2, HSFGFR3EX, JTK4

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.8
PP2 >100,000
AG1478 >100,000
PDGFR I-III 77,000
D-64406 5,400
Scientific Information
P22607 (http://www.uniprot.org/uniprot/P22607)

Screening Location
Malvern, USA

Reference compound IC50 for FGFR3 (V555M)
Reference compound IC50 for FGFR3 (V555M)

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