Activity-based FGFR4 V550E kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FGFR4 V550E
Enzyme
Human FGFR4 V550E, mutant
HGNC
FGFR4 (V550E)
Manning ID
FGFR4
Synonym
TKF, JTK2, MGC20292, FGFR4 (V550E)
UniProt
P22455 (FGFR4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 190 |
| Sorafenib | 58000 |
| Sunitinib | 48000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FGFR4 V550E |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – FGFR4 V550E (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1771-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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