Activity-based FGFR4 (V550E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR4 (V550E)
Enzyme
Human FGFR4 (V550E)
HGNC
FGFR4 (V550E)
Synonym
TKF, JTK2, MGC20292
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3,700 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | >100,000 |
Scientific Information
P22455 (http://www.uniprot.org/uniprot/P22455)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR4 (V550E)
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