Activity-based FLT3 (F691L) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FLT3 (F691L)
Enzyme
Human FLT3 (F691L)
HGNC
FLT3 (F691L)
Synonym
FL cytokine receptor [Precursor], Tyrosine-protein kinase receptor FLT3,
Stem cell tyrosine kinase 1, STK-1, Flk-2
Stem cell tyrosine kinase 1, STK-1, Flk-2
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)
Assay Properties
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| PP2 | 37,000 |
| AG1478 | 5,800 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 320 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for FLT3 (F691L)
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