FLT3 (F594_R595insR) Kinase Assay Service

Activity-based FLT3 (F594_R595insR) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT3 (F594_R595insR)

Enzyme
Human FLT3 (F594_R595insR)

HGNC
FLT3 (F594_R595insR)

Synonym
FLK2, STK1, CD135

Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.4
PP2 3,400
AG1478 130
PDGFR I-III 64
D-64406 35
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)

Screening Location
Malvern, USA

Reference compound IC50 for FLT3 (F594_R595insR)
Reference compound IC50 for FLT3 (F594_R595insR)

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