FLT3 (ITD)-NPOS Kinase Assay Service

Activity-based FLT3 (ITD)-NPOS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT3 (ITD)-NPOS

Enzyme
Human FLT3 (ITD) (L610_E611insCSSDNEYFYVDFREYEYDLKWEFPRENL)

HGNC
FLT3 (ITD)-NPOS

Synonym
FLK2, STK1, CD135

Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.1
PP2 10,000
AG1478 730
PDGFR I-III 310
D-64406 55
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)

Screening Location
Malvern, USA

Reference compound IC50 for FLT3 (ITD)-NPOS
Reference compound IC50 for FLT3 (ITD)-NPOS

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