Activity-based FLT3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FLT3
Enzyme
Human FLT3, wt
HGNC
FLT3
Manning ID
FLT3
Synonym
FLK2, STK1, CD135, FLT3, STK-1, FLT3 wt
UniProt
P36888 (FLT3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1 |
| Sorafenib | 110 |
| Sunitinb | 18 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FLT3 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – FLT3 |
| Product Number | 0776-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF