FLT3 Kinase Assay Service

Activity-based FLT3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT3

Enzyme
Human FLT3

HGNC
FLT3

Synonym
FLK2, STK1, CD135

Substrates
Peptide substrate, [EAIYAAPFAKKK], 5 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 2.2
PP2 2,900
AG1478 320
PDGFR I-III 302
D-64406 56.5
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)

Screening Location
Malvern, USA

Reference compound IC50 for FLT3
Reference compound IC50 for FLT3

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