FLT4/VEGFR3 Kinase Assay Service

Activity-based FLT4/VEGFR3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT4

Enzyme
Human FLT4

HGNC
FLT4

Synonym
VEGFR3, PCL

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µm

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3.2
PP2 900
AG1478 2,200
PDGFR I-III 15,600
D-64406 2,600
Scientific Information
P35916 (http://www.uniprot.org/uniprot/P35916)

Screening Location
Malvern, USA

Reference compound IC50 for FLT4/VEGFR3
Reference compound IC50 for FLT4/VEGFR3

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