Activity-based FMS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FMS
Enzyme
Human FMS
HGNC
CSF1R
Synonym
C-FMS, CD115, CSF-1-R, CSFR, FIM2, FMS
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.7 |
| PP2 | 2,200 |
| AG1478 | >20,000 |
| PDGFR I-III | 1,900 |
| D-64406 | 7,400 |
Scientific Information
P07333 (http://www.uniprot.org/uniprot/P07333)
Screening Location
Malvern, USA
Reference compound IC50 for FMS
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