Activity-based FYN Y531F kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FYN Y531F
Enzyme
Human FYN Y531F, mutant
HGNC
FYN (Y531F)
Manning ID
FYN
Synonym
FYN, SLK, SYN, p59-FYN, cFYN, p59-Fyn, FYN Y531F, FYN (Y531F)
UniProt
P06241 (FYN_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 50 |
| Sorafenib | 10000 |
| Sunitinb | 530 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FYN Y531F |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – FYN Y531F |
| Product Number | 1457-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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