Activity-based G9a Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
G9a
Human G9a
Histone-lysine N-methyltransferase EHMT2, Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8 Protein G9a, Lysine N-methyltransferase 1C, EHMT2, BAT8, C6orf30, G9A, KMT1C, NG36
Histone H3, or H3 (1-21) peptide, 5 µM
SAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Enzymatic activity
| Compounds | IC50 (nM) With Histone H3 substrate |
|---|---|
| SAH | 2,300 |
| Sinefungin | 94,000 |
| BIX 01294 | >200,000 |
| Chaetocin | 6,000 |
| Compounds | IC50 (nM) With H3 (1-21) peptide substrate |
| SAH | 760 |
| Sinefungin | 8,300 |
| BIX 01294 | 5,300 |
| Chaetocin | 8,000 |
Q96KQ7 (http://www.uniprot.org/uniprot/Q96KQ7)
Malvern, PA, USA
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for G9a/Histone H3
Reference Compound IC50 for G9a/H3 (1-21)
