GAK Kinase Activity Assay Service

Activity-based GAK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

GAK

Enzyme

Human GAK, wt

HGNC

GAK

Manning ID

GAK

Synonym

auxilin2, DNAJC26, MGC99654

UniProt
Kinase Type

Protein kinase

Kinase Group

Other

Assay Properties
Assay Format

33PanQinaseTM

Substrates

ERK2 K54R, ATP

Reaction

Substrate + 33P-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 1600
Sorafenib 2600
Sunitinib >10000
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice GAK
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – GAK
(Activity verified by 33PanQinaseTM-Assay)
Product Number 1974-0000-1
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