Activity-based GLP Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
GLP
Enzyme
Human GLP
Synonym
Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, EUHMTASE1, GLP, KIAA1876, KMT1D
Substrates
Histone H3, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 350 |
| Sinefungin | 13,000 |
| BIX 01294 | 74,000 |
| Chaetocin | 9,300 |
Scientific Information
Q9H9B1 (http://www.uniprot.org/uniprot/Q9H9B1)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for GLP
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