Glucocorticoid Nuclear Receptor (GR) Biochemical Binding Assay for compound screening and profiling via fluorescence polarization.
Target Overview
CAT#
GR FP
Nuclear Receptor Target
Glucocorticoid Receptor
Source
Receptor preparation from insect cells expressing full length glucocorticoid receptors
Scientific Information
NR3C1 (https://www.uniprot.org/uniprotkb/P04150/entry)
Assay Properties
Protocol
Fluorescently labeled tracer binding to GR preparation ± competing ligand; fluorescence polarization measurement on Envision plate reader
Readout
mP derived from S and P polarized light measurements
Reference Compound IC50
| Compound IC50 (nM) | Tracer (nM) |
|---|---|
| Dexamethasone (15.5) | Fluomone TM GS Red (1.4) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for GR
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