GRK2 Kinase Assay Service

Activity-based GRK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
GRK2

Enzyme
Human GRK2

HGNC
GRK2

Synonym
ADRBK1

Substrates
Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 802
Ro 31-8220 17,400
H-89 10,800
Scientific Information
P25098 (http://www.uniprot.org/uniprot/P25098)

Screening Location
Malvern, USA

Reference compound IC50 for GRK2
Reference compound IC50 for GRK2

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