Activity-based GRK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GRK7
Enzyme
Human GRK7
HGNC
GRK7
Synonym
GPRK7
Substrates
Casein, 1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.5 |
| Ro 31-8220 | 1,500 |
| GW5074 | 40,900 |
| H-89 | 15,000 |
Scientific Information
Q8WTQ7 (http://www.uniprot.org/uniprot/Q8WTQ7)
Screening Location
Malvern, USA
Reference compound IC50 for GRK7
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