Activity-based GSK3a kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GSK3a
Enzyme
Human GSK3a
HGNC
GSK3A
Synonym
GSK3alpha
Substrates
Peptide substrate, [YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.6 |
| Ro 31-8220 | 0.93 |
| GW 5074 | 3,900 |
Scientific Information
P49840 (http://www.uniprot.org/uniprot/P498410)
Screening Location
Malvern, USA
Reference compound IC50 for GSK3a
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