Activity-based GSK3alpha kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
GSK3alpha
Enzyme
Human GSK3alpha, wt
HGNC
GSK3A
Manning ID
GSK3A
Synonym
GSK-alpha, GSK3alpha, GSK3A, GSK3-alpha, GSK3a
UniProt
P49840 (GSK3A_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
SUPT5 754-837, ATP
Reaction
SUPT5 754-837 + 33P-ATP = 33P-SUPT5 754-837 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 41 |
| Sorafenib | 10000 |
| Sunitinib | 63000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | GSK3alpha |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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