Activity-based GSK3b (C199A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GSK3b (C199A)
Enzyme
Human GSK3b (C199A)
HGNC
GSK3b (C199A)
Synonym
Glycogen synthase kinase-3 beta
Scientific Information
P49841 (http://www.uniprot.org/uniprot/P49841)
Assay Properties
Substrates
Peptide substrate, [YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 12 |
| Ro 31-8220 | 3.8 |
| GW5074 | 1,100 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for GSK3b (C199A)
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