H1 histaminergic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
H1 Binding
GPCR Target
Human H1
Source
Membrane preparation from HEK293 cells stably transfected with human H1 receptor
Scientific Information
HRH1 (https://www.uniprot.org/uniprotkb/P35367/entry)
Assay Properties
Protocol
Radioligand filter binding to H1 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Pyrilamine (8.4) | Pyrilamine (5) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for H1
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