Activity-based HGK/MAP4K4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
HGK
Enzyme
Human HGK
HGNC
MAP4K4
Synonym
HGK
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.17 |
| Ro 31-8220 | 74.3 |
| GW 5074 | 11.5 |
| H-89 | 29,500 |
| Rottlerin | 8,200 |
Scientific Information
O95819 (http://www.uniprot.org/uniprot/O95819)
Screening Location
Malvern, USA
Reference compound IC50 for HGK/MAP4K4
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