HIPK3 Kinase Assay Service

Activity-based HIPK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
HIPK3

Enzyme
Human HIPK3

HGNC
HIPK3

Synonym
ANPK, DYRK6, FIST, FIST3, PKY, YAK1

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4,600
Ro 31-8220 2,800
GW 5074 3,200
Rottlerin 14,000
Scientific Information
Q9H422 (http://www.uniprot.org/uniprot/Q9H422)

Screening Location
Malvern, USA

Reference compound IC50 for HIPK3
Reference compound IC50 for HIPK3

PDF