Activity-based HIPK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
HIPK4
Enzyme
Human HIPK4
HGNC
HIPK4
Synonym
FLJ32818
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 621 |
| Ro 31-8220 | 961 |
| GW 5074 | 882 |
| Rottlerin | 61,400 |
Scientific Information
Q8NE63 (http://www.uniprot.org/uniprot/Q8NE63)
Screening Location
Malvern, USA
Reference compound IC50 for HIPK4
PDF