HPK1/MAP4K1 Kinase Assay Service

Activity-based HPK1/MAP4K1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
HPK1/MAP4K1

Enzyme
Human HPK1/MAP4K1

HGNC
MAP4K1

Synonym
HPK1

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
Ro 31-8220 16
GW 5074 3,100
H-89 35,000
Rottlerin >50,000
Scientific Information
Q92918 (http://www.uniprot.org/uniprot/Q92918)

Screening Location
Malvern, USA

Reference compound IC50 for HPK1/MAP4K1
Reference compound IC50 for HPK1/MAP4K1

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