Activity-based ICK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ICK
Enzyme
Human ICK, wt
HGNC
CILK
Manning ID
ICK
Synonym
KIAA0936, LCK2, MGC46090, MRK
UniProt
Q9UPZ9 (CILK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
Substrate + 33P-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 90 |
| Sorafenib | >10000 |
| Sunitinib | 8600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ICK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ICK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1986-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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