IDH1 (G97D) Isocitrate Dehydrogenase Assay Service

IDH1 (G97D) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.

Target Overview
CAT#

IDH1 (G97D)

Enzyme

Human IDH1 (G97D) Mutant

Synonym

Isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Scientific Information

O75874 (http://www.uniprot.org/uniprot/O75874)

Assay Properties
Substrate

a-ketoglutarate, 400 µM
NADPH, 15 µM

Reaction

aKG + NADPH = 2HG + NADP

Detection

Resorufin fluorescence (diaphorase/resazurin coupled reaction)

Reference Compound IC50s
CompoundIC50 (nM)
AG-120 514
AGI-5198 16000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.

Reference compound IC50 for IDH1 (G97D)