IDH1 (R100Q) Isocitrate Dehydrogenase Assay Service

IDH1 (R100Q) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Enzyme

Human IDH1 (R100Q) mutant

Synonym

Isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Substrate

5 mM α-ketoglutarate, 15 µM NADPH

Reaction

aKG + NADPH = 2HG + NADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
AG-120 150
AGI-5198 >50,000
Scientific Information

O75874 (http://www.uniprot.org/uniprot/O75874)

Screening Location

Malvern, USA

Reference compound IC50 for IDH1 (R100Q)

Reference compound IC50 for IDH1 (R100Q)