IDH1 (R100Q) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH1 (R100Q)
Enzyme
Human IDH1 (R100Q) mutant
Synonym
Isocitrate dehydrogenase [NADP] cytoplasmic, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase
Scientific Information
O75874 (http://www.uniprot.org/uniprot/O75874)
Assay Properties
Substrate
a-ketoglutarate, 5 mM
NADP, 15 µM
Reaction
aKG + NADPH = 2HG + NADP
Detection
Resorufin fluorescence (diaphorase/resazurin coupled reaction)
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| AG-120 | 512 |
| AGI-5198 | >>50000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.
Reference compound IC50 for IDH1 (R100Q)
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