IDH1 (R132C) Isocitrate Dehydrogenase Assay Service

IDH1 (R132C) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
CAT#

IDH1 (R132C)

Enzyme

Human IDH1 (R132C) mutant

Synonym

Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42), NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Substrate

0.5 mM α-ketoglutarate, 15 µM NADPH

Reaction

aKG + NADPH = 2HG + NADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
AGI-5198 5.8
IDH-C227 1.2
AG-120 8.2
Scientific Information

O75874 (http://www.uniprot.org/uniprot/O75874)

Screening Location

Malvern, USA

Reference compound IC50 for IDH1 (R132C)

Reference compound IC50 for IDH1 (R132C)