IDH1 WT Isocitrate Dehydrogenase Assay Service

IDH1 WT Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
CAT#

IDH1 WT

Enzyme

Human IDH1 wild type

Synonym

Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42), NADP(+)-specific ICDH, Oxalosuccinate decarboxylase)

Substrate

65 µM isocitrate, 50 µM NADP

Reaction

Isocitrate + NADP = aKG + NADPH

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
AGI-5198 >20000
IDH-C227 >20000
Scientific Information

O75874 (http://www.uniprot.org/uniprot/O75874 )

Screening Location

Malvern, USA

Reference compound inhibition of IDH1 WT

Reference compound inhibition of IDH1 WT