IDH2 (R140K) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#
IDH2 (R140K)
Enzyme
Human IDH2 (R140K) mutant
Synonym
Isocitrate dehydrogenase [NADP], mitochondrial, ICD-M, Oxalosuccinate decarboxylase)
Substrate
5 mM α-ketoglutarate, 15 µM NADPH
Assay Properties
Reaction
aKG + NADPH = 2HG + NADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| IDH2-C100 | 5.0 |
| AG-221 | 44 |
| AGI-6780 | 3.6 |
Scientific Information
P48735 (http://www.uniprot.org/uniprot/P48735)
Screening Location
Malvern, USA
Reference compound IC50 for IDH2 (R140K)
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