IDH2 (R140Q) Isocitrate Dehydrogenase Assay Service

IDH2 (R140Q) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
CAT#

IDH2 (R140Q)

Enzyme

Human IDH2 (R140Q) mutant

Synonym

Isocitrate dehydrogenase [NADP], mitochondrial, ICD-M, Oxalosuccinate decarboxylase

Substrate

8 mM α-ketoglutarate, 15 µM NADPH

Reaction

aKG + NADPH = 2HG + NADP

Readout

Enzymatic activity

Reference Compound IC50s
Compound IC50 (nM)
IDH2-C100 4.2
AG-221 140
AGI-6780 8.4
Scientific Information

P48735 (http://www.uniprot.org/uniprot/P48735)

Screening Location

Malvern, USA

Reference compound IC50 for IDH2 (R140Q)

Reference compound IC50 for IDH2 (R140Q)