IDH2 (R172Q) Isocitrate Dehydrogenase Assay Service

IDH2 (R172Q) Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
CAT#

IDH2 (R172Q)

Enzyme

Human IDH2 (R172Q) mutant

Synonym

Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42); ICD-M; Oxalosuccinate decarboxylase

Substrate

50 µM α-ketoglutarate, 15 µM NADPH

Reaction

aKG + NADPH = 2HG + NADP

Readout

Enzymatic activity

Reference Compound IC50s
Compound IC50 (nM)
IDH2-C100 83
AG-221 19
AGI-6780 10
Scientific Information

P48735 (http://www.uniprot.org/uniprot/P48735)

Screening Location

Malvern, USA

Reference compound IC50 for IDH2 (R172Q)

Reference compound IC50 for IDH2 (R172Q)