IDH2 wt Isocitrate Dehydrogenase Assay Service

IDH2 wt Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
CAT#

IDH2 wt

Enzyme

Human IDH2 wild type

Synonym

Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42); ICD-M; Oxalosuccinate decarboxylase)

Substrate

150 µM isocitrate , 25 µM NADP

Reaction

isocitrate + NADP = a-KG + NADPH

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IDH-C100 227
Scientific Information

P48735 (http://www.uniprot.org/uniprot/P48735 )

Screening Location

Malvern, USA

Reference compound IC50 for IDH2 WT

Reference compound IC50 for IDH2 WT