IDH2 WT Isocitrate Dehydrogenase Assay Service

IDH2 WT Isocitrate Dehydrogenase Assay for compound screening and profiling via Diaphorase/Resazurin-coupled assay for quantification of enzyme activity.
Target Overview
CAT#

IDH2 WT

Enzyme

Human IDH2 wild type

Synonym

Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42); ICD-M; Oxalosuccinate decarboxylase

Scientific Information

P48735 (http://www.uniprot.org/uniprot/P48735)

Assay Properties
Substrate

150 µM isocitrate , 25 µM NADP

Reaction

isocitrate + NADP = a-KG + NADPH

Detection

Resorufin fluorescence (diaphorase/resazurin coupled reaction)

Reference Compound IC50s
Compounds IC50 (nM)
IDH-C100 1800
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Isocitrate Dehydrogenase Assay Services for Drug Discovery.

Reference compound IC50 for IDH2 WT