IKKa/CHUK Kinase Assay Service

Activity-based IKKa/CHUK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
IKKa/CHUK

Enzyme
Human IKKa/CHUK

HGNC
CHUK

Synonym
IKBKA, IKK1, IKKA, NFKBIKA, TCF16, IKK-alpha

Substrates
Peptide substrate, [KKKKERLLDDRHDSGLDSMKDEE], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 280
Ro 31-8220 20,000
GW5074 >20,000
Rottlerin 4,200
Scientific Information
O15111 (http://www.uniprot.org/uniprot/O15111)

Screening Location
Malvern, USA

Reference compound IC50 for IKKa/CHUK
Reference compound IC50 for IKKa/CHUK

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