IKKb/IKBKB Kinase Assay Service

Activity-based IKKb/IKBKB kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

IKKb/IKBKB

Enzyme

Human IKKb/IKBKB

HGNC

IKBKB

Synonym

IKK2, IKKB, NFKBIKB, IKK-beta

Substrates

Peptide substrate, [KKKKERLLDDRHDSGLDSMKDEE], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	405

Scientific Information

O14920 (http://www.uniprot.org/uniprot/O14920)

Screening Location

Malvern, USA

Reference compound IC50 for IKKb/IKBKB