Activity-based IKKe/IKBKE kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
IKKe
Enzyme
Human IKKe
HGNC
IKBKE
Synonym
IKKI, IKK-i, KIAA0151, IKK-epsilon
Substrates
Casein, 2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Staurosporine | 0.3 |
Ro 31-8220 | 3,200 |
GW 5074 | 72,000 |
Scientific Information
Q14164 (http://www.uniprot.org/uniprot/Q14164)
Screening Location
Malvern, USA
Reference compound IC50 for IKKepsilon/IKBKE
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