Activity-based IKKepsilon kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
IKKepsilon
Enzyme
Human IKKepsilon, wt
HGNC
IKBKE
Manning ID
IKKe
Synonym
IKK-epsilon, IKKE, IKKI, IKK-i, KIAA0151, IKBKE, IKKepsilon, IKKe/IKBKE
UniProt
Q14164 (IKKE_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7 |
| Sorafenib | 10000 |
| Sunitinb | 1900 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | IKKepsilon |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – IKKepsilon |
| Product Number | 0320-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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