Activity-based IR kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
IR
Enzyme
Human IR
HGNC
INSR
Synonym
Insulin receptor, INSR, CD220
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 41 |
| PP2 | 72,000 |
| AG1478 | >100,000 |
| PDGFR I-III | >100,000 |
| D-64406 | >100,000 |
Scientific Information
P06213 (http://www.uniprot.org/uniprot/P06213)
Screening Location
Malvern, USA
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