IRAK1 Kinase Assay Service - Reaction Biology

Activity-based IRAK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

IRAK1

Enzyme

Human IRAK1

HGNC

IRAK1

Synonym

Pelle

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	48

Scientific Information

P51617 (http://www.uniprot.org/uniprot/P51617)

Screening Location

Malvern, USA

Reference compound IC50 for IRAK1