IRAK4 Kinase Assay Service

Activity-based IRAK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
IRAK4

Enzyme
Human IRAK4

HGNC
IRAK4

Synonym
REN64

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.6
Ro 31-8220 950
GW 5074 10,000
H-89 >20,000
Scientific Information
Q9NWZ3 (http://www.uniprot.org/uniprot/Q9NWZ3)

Screening Location
Malvern, USA

Reference compound IC50 for IRAK4
Reference compound IC50 for IRAK4

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