Activity-based ITK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ITK
Enzyme
Human ITK
HGNC
ITK
Synonym
EMT 2, LYK 2, PSCTK2 2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6.4 |
| PP2 | >20,000 |
| AG1478 | 19,000 |
| PDGFR I-III | >20,000 |
| D-64406 | 3,000 |
Scientific Information
Q08881 (http://www.uniprot.org/uniprot/Q08881)
Screening Location
Malvern, USA
Reference compound IC50 for ITK
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