Activity-based JAK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
JAK1
Enzyme
Human JAK1, wt
HGNC
JAK1
Manning ID
JAK1
Synonym
JAK1A, JAK1B, JTK3, JAK1, JAK1 aa583-1154 wt
UniProt
P23458 (JAK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-IRStide, ATP
Reaction
RBER-IRStide + 33P-ATP = 33P-RBER-IRStide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11 |
| Sorafenib | 6500 |
| Sunitinb | 11000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | JAK1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – JAK1 |
| Product Number | 1480-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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